@article{BARRIAS2025111036,

title = {Newly developed QCM-DNA biosensor for SNP detection in small DNA fragments: A wine authenticity case study},

author = {Sara Barrias and José R. Fernandes and Paula Martins-Lopes},

url = {https://www.sciencedirect.com/science/article/pii/S0956713524007539},

doi = {https://doi.org/10.1016/j.foodcont.2024.111036},

issn = {0956-7135},

year  = {2025},

date = {2025-03-01},

urldate = {2025-03-01},

journal = {Food Control},

volume = {169},

pages = {111036},

abstract = {We propose a QCM-DNA biosensor for single nucleotide polymorphism (SNP) detection in samples of differing complexity. An optimized protocol is presented, focusing on parameters affecting probe immobilization and hybridization efficiency. Our results led to the implementation of thiolated probe reduction with TCEP, followed by immobilization in PBS buffer containing MgCl2. The biosensor exhibited an enhanced specificity at 37 °C, achieving detection of single mismatches using synthetic targets. Using real samples, we applied the biosensor in a wine authenticity assessment context. The addition of dithiothreitol improved stability and reproducibility when testing wine DNA samples. The QCM-DNA biosensor was able to specifically detect complementary DNA in leaf and wine DNA samples, distinguishing between samples with two heterozygous mismatches. The biosensor solely depends on DNA extraction, basic instrumentation, and reagents, without requiring PCR or signal amplification strategies. Our findings show the biosensor potential for applications in wine authenticity assessment and other fields requiring complex analysis in undemanding settings.},

keywords = {Aptamer, DNA, Grapevine, Microbalance, openQCM Q-1, Piezoelectric, QCM-D, Quartz Crystal Microbalance, SNP, Wine},

pubstate = {published},

tppubtype = {article}

}

@article{lino2023development,

title = {Development of a QCM-based biosensor for the detection of non-small cell lung cancer biomarkers in liquid biopsies},

author = {Catarina Lino and Sara Barrias and Raquel Chaves and Filomena Adega and José Ramiro Fernandes and Paula Martins-Lopes},

url = {https://www.sciencedirect.com/science/article/pii/S0039914023003752},

doi = {https://doi.org/10.1016/j.talanta.2023.124624},

year  = {2023},

date = {2023-05-04},

urldate = {2023-05-04},

journal = {Talanta},

pages = {124624},

publisher = {Elsevier},

abstract = {Lung cancer is the main malignant cancer reported worldwide, with one of the lowest survival rates. Deletions in the Epidermal Growth Factor Receptor (EGFR) gene are often associated with non-small cell lung cancer (NSCLC), a common subtype of lung cancer. The detection of such mutations provides key information for the diagnosis and treatment of the disease; therefore, the early screening of such biomarkers is of vital importance. The need for fast, reliable, and early detection means applied to NSCLC has led to the development of highly sensitive devices that can detect cancer-associated mutations. Such devices, known as biosensors, are a promising alternative to more conventional detection methods and can potentially alter the way cancer is diagnosed and treated. In this study, we report the development of a DNA-based biosensor, namely a quartz crystal microbalance (QCM), applied to the detection of NSCLC, from liquid biopsies samples. The detection, as is the case of most DNA biosensors, is based on the hybridization between the NSCLC-specific probe and the sample DNA (containing specific mutations associated with NSCLC). The surface functionalization was performed with a blocking agent (dithiothreitol) and thiolated-ssDNA strands. The biosensor was able to detect specific DNA sequences in both synthetic and real samples. Aspects such as reutilization and regeneration of the QCM electrode were also studied.},

key = {QCM, Quartz Crystal Microbalance, openQCM Q-1, DNA, cancer, biosensor, blood plasma},

keywords = {biosensors, blood plasma, Cancer, DNA, openQCM Q-1, QCM, Quartz Crystal Microbalance},

pubstate = {published},

tppubtype = {article}

}