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Scientific Papers
Publications citing the applications of openQCM (by Novaetech S.r.l.) instruments and accessories in scientific research.
The list of scientific papers published on the most important journals showing the usage of openQCM in several scientific fields, such as thin film deposition, chemical sensors, biological research and biosensors.
Because of the large number of publications, we are reorganizing everything by subject areas. This will take some time. Thank you for your patience
2024
Forinová, Michala; Pilipenco, Alina; Lynn, N. Scott; Obořilová, Radka; Šimečková, Hana; Vrabcová, Markéta; Spasovová, Monika; Jack, Rachael; Horák, Petr; Houska, Milan; Skládal, Petr; Šedivák, Petr; Farka, Zdeněk; Vaisocherová-Lísalová, Hana
A reusable QCM biosensor with stable antifouling nano-coating for on-site reagent-free rapid detection of E. coli O157:H7 in food products Journal Article
In: Food Control, vol. 165, pp. 110695, 2024, ISSN: 0956-7135.
Abstract | Links | BibTeX | Tags: Antifouling coating, O157:H7 detection, On-site analysis, openQCM Q-1, QCM, QCM biosensor, Quartz Crystal Microbalance, Reusability
@article{FORINOVA2024110695b,
title = {A reusable QCM biosensor with stable antifouling nano-coating for on-site reagent-free rapid detection of E. coli O157:H7 in food products},
author = {Michala Forinová and Alina Pilipenco and N. Scott Lynn and Radka Obořilová and Hana Šimečková and Markéta Vrabcová and Monika Spasovová and Rachael Jack and Petr Horák and Milan Houska and Petr Skládal and Petr Šedivák and Zdeněk Farka and Hana Vaisocherová-Lísalová},
url = {https://www.sciencedirect.com/science/article/pii/S0956713524004122},
doi = {https://doi.org/10.1016/j.foodcont.2024.110695},
issn = {0956-7135},
year = {2024},
date = {2024-11-01},
urldate = {2024-11-15},
journal = {Food Control},
volume = {165},
pages = {110695},
abstract = {Numerous biosensors have shown exceptional analytical performance under laboratory conditions, yet only a few are capable of on-site use with complex, non-model samples while exhibiting reliable analytical performance. Here, we present a new portable biosensor for the rapid (30 min) and accurate detection of bacterial agents in “real-world” food samples, which are originally in either solid or liquid form. The biosensor combines well-established quartz crystal microbalance (QCM) technology, with innovative terpolymer brush nano-coatings on the sensing surface to efficiently reduce non-specific fouling from food samples. Following reagent-free sample preparation, where solid food samples are homogenized, we validated the sensor's detection capabilities on native pathogenic Escherichia coli O157:H7 (E. coli O157:H7) in hamburgers, Czech dumplings, and milk. We achieved limits of detection (LOD), as low as 7.5 × 102 CFU/mL in milk, a value approaching fundamental QCM limits, using a simple direct detection assay format. The biosensor's exceptional reusability was demonstrated through 60 sequential hamburger sample injections, resulting in only a minor LOD shift toward the end of series. A 10-min sonication treatment during sample preparation significantly enhanced sensitivity for E. coli O157:H7 in hamburgers and dumplings, yielding LODs as low as 3.1 × 10³ CFU/mL and 2.6 × 10⁴ CFU/mL, respectively. For on-site analysis, we integrated the nano-coated sensing chip into a custom-built four-channel portable QCM biosensor with an optimized microfluidic system, which can be produced on a scale suitable for practical deployment.},
keywords = {Antifouling coating, O157:H7 detection, On-site analysis, openQCM Q-1, QCM, QCM biosensor, Quartz Crystal Microbalance, Reusability},
pubstate = {published},
tppubtype = {article}
}
Numerous biosensors have shown exceptional analytical performance under laboratory conditions, yet only a few are capable of on-site use with complex, non-model samples while exhibiting reliable analytical performance. Here, we present a new portable biosensor for the rapid (30 min) and accurate detection of bacterial agents in “real-world” food samples, which are originally in either solid or liquid form. The biosensor combines well-established quartz crystal microbalance (QCM) technology, with innovative terpolymer brush nano-coatings on the sensing surface to efficiently reduce non-specific fouling from food samples. Following reagent-free sample preparation, where solid food samples are homogenized, we validated the sensor's detection capabilities on native pathogenic Escherichia coli O157:H7 (E. coli O157:H7) in hamburgers, Czech dumplings, and milk. We achieved limits of detection (LOD), as low as 7.5 × 102 CFU/mL in milk, a value approaching fundamental QCM limits, using a simple direct detection assay format. The biosensor's exceptional reusability was demonstrated through 60 sequential hamburger sample injections, resulting in only a minor LOD shift toward the end of series. A 10-min sonication treatment during sample preparation significantly enhanced sensitivity for E. coli O157:H7 in hamburgers and dumplings, yielding LODs as low as 3.1 × 10³ CFU/mL and 2.6 × 10⁴ CFU/mL, respectively. For on-site analysis, we integrated the nano-coated sensing chip into a custom-built four-channel portable QCM biosensor with an optimized microfluidic system, which can be produced on a scale suitable for practical deployment.
Forinová, Michala; Pilipenco, Alina; Lynn, N. Scott; Obořilová, Radka; Šimečková, Hana; Vrabcová, Markéta; Spasovová, Monika; Jack, Rachael; Horák, Petr; Houska, Milan; Skládal, Petr; Šedivák, Petr; Farka, Zdeněk; Vaisocherová-Lísalová, Hana
A reusable QCM biosensor with stable antifouling nano-coating for on-site reagent-free rapid detection of E. coli O157:H7 in food products Journal Article
In: Food Control, pp. 110695, 2024, ISSN: 0956-7135.
Abstract | Links | BibTeX | Tags: Antifouling coating, O157:H7 detection, On-site analysis, openQCM Q-1, QCM, QCM biosensor, Reusability
@article{FORINOVA2024110695,
title = {A reusable QCM biosensor with stable antifouling nano-coating for on-site reagent-free rapid detection of E. coli O157:H7 in food products},
author = {Michala Forinová and Alina Pilipenco and N. Scott Lynn and Radka Obořilová and Hana Šimečková and Markéta Vrabcová and Monika Spasovová and Rachael Jack and Petr Horák and Milan Houska and Petr Skládal and Petr Šedivák and Zdeněk Farka and Hana Vaisocherová-Lísalová},
url = {https://www.sciencedirect.com/science/article/pii/S0956713524004122},
doi = {https://doi.org/10.1016/j.foodcont.2024.110695},
issn = {0956-7135},
year = {2024},
date = {2024-07-01},
urldate = {2024-01-01},
journal = {Food Control},
pages = {110695},
abstract = {Numerous biosensors have shown exceptional analytical performance under laboratory conditions, yet only a few are capable of on-site use with complex, non-model samples while exhibiting reliable analytical performance. Here, we present a new portable biosensor for the rapid (30 min) and accurate detection of bacterial agents in "real-world" food samples, which are originally in either solid or liquid form. The biosensor combines well-established quartz crystal microbalance (QCM) technology, with innovative terpolymer brush nano-coatings on the sensing surface to efficiently reduce non-specific fouling from food samples. Following reagent-free sample preparation, where solid food samples are homogenized, we validated the sensor's detection capabilities on native pathogenic Escherichia coli O157:H7 (E. coli O157:H7) in hamburgers, Czech dumplings, and milk. We achieved limits of detection (LOD), as low as 7.5 × 102 CFU/mL in milk, a value approaching fundamental QCM limits, using a simple direct detection assay format. The biosensor's exceptional reusability was demonstrated through 60 sequential hamburger sample injections, resulting in only a minor LOD shift toward the end of the series. A 10-minute sonication treatment during sample preparation significantly enhanced sensitivity for E. coli O157:H7 in hamburgers and dumplings, yielding LODs as low as 3.1 × 10³ CFU/mL and 2.6 × 10⁴ CFU/mL, respectively. For on-site analysis, we integrated the nano-coated sensing chip into a custom-built four-channel portable QCM biosensor with an optimized microfluidic system, which can be produced on a scale suitable for practical deployment.},
keywords = {Antifouling coating, O157:H7 detection, On-site analysis, openQCM Q-1, QCM, QCM biosensor, Reusability},
pubstate = {published},
tppubtype = {article}
}
Numerous biosensors have shown exceptional analytical performance under laboratory conditions, yet only a few are capable of on-site use with complex, non-model samples while exhibiting reliable analytical performance. Here, we present a new portable biosensor for the rapid (30 min) and accurate detection of bacterial agents in "real-world" food samples, which are originally in either solid or liquid form. The biosensor combines well-established quartz crystal microbalance (QCM) technology, with innovative terpolymer brush nano-coatings on the sensing surface to efficiently reduce non-specific fouling from food samples. Following reagent-free sample preparation, where solid food samples are homogenized, we validated the sensor's detection capabilities on native pathogenic Escherichia coli O157:H7 (E. coli O157:H7) in hamburgers, Czech dumplings, and milk. We achieved limits of detection (LOD), as low as 7.5 × 102 CFU/mL in milk, a value approaching fundamental QCM limits, using a simple direct detection assay format. The biosensor's exceptional reusability was demonstrated through 60 sequential hamburger sample injections, resulting in only a minor LOD shift toward the end of the series. A 10-minute sonication treatment during sample preparation significantly enhanced sensitivity for E. coli O157:H7 in hamburgers and dumplings, yielding LODs as low as 3.1 × 10³ CFU/mL and 2.6 × 10⁴ CFU/mL, respectively. For on-site analysis, we integrated the nano-coated sensing chip into a custom-built four-channel portable QCM biosensor with an optimized microfluidic system, which can be produced on a scale suitable for practical deployment.
2023
Forinová, Michala; Seidlová, Anna; Pilipenco, Alina; Jr, N Scott Lynn; Obořilová, Radka; Farka, Zdeněk; Skládal, Petr; Saláková, Alena; Spasovová, Monika; Houska, Milan; others,
In: Current Research in Biotechnology, pp. 100166, 2023.
Abstract | Links | BibTeX | Tags: Antifouling coating, biosensors, Cultivation-based methods, openQCM Q-1, QCM-D, Quartz Crystal Microbalance, S. aureus
@article{forinova2023comparative,
title = {A Comparative Assessment of a Piezoelectric Biosensor Based on a New Antifouling Nanolayer and Cultivation Methods: Enhancing S. aureus Detection in Fresh Dairy Products},
author = {Michala Forinová and Anna Seidlová and Alina Pilipenco and N Scott Lynn Jr and Radka Obořilová and Zdeněk Farka and Petr Skládal and Alena Saláková and Monika Spasovová and Milan Houska and others},
url = {https://www.sciencedirect.com/science/article/pii/S2590262823000485},
doi = {https://doi.org/10.1016/j.crbiot.2023.100166},
year = {2023},
date = {2023-11-23},
urldate = {2023-11-23},
journal = {Current Research in Biotechnology},
pages = {100166},
publisher = {Elsevier},
abstract = {Ensuring dairy product safety demands rapid and precise Staphylococcus aureus (S. aureus) detection. Biosensors show promise, but their performance is often demonstrated in model samples using non-native pathogens and has never been studied towards S. aureus detection in naturally contaminated samples. This study addresses the gap by directly comparing results taken with a novel piezoelectric biosensor, capable of one-step detection, with four conventional cultivation-based methods. Our findings reveal that this biosensor, based on an antifouling nanolayer-coated biochip, exhibits exceptional resistance to biofouling from unprocessed dairy products and is further capable of specific S. aureus detection. Notably, it performed comparably to Petrifilm and Baird-Parker methods but delivered results in only 30 min, bringing a substantial reduction from the 24 h required by cultivation-based techniques. Our study also highlights differences in the performance of cultivation methods when analyzing artificially spiked versus naturally contaminated foods. These findings underline the potential of antifouling biosensors as efficient reliable tools for rapid, cost-effective, point-of-care testing, enhancing fresh dairy product safety and S. aureus detection.},
keywords = {Antifouling coating, biosensors, Cultivation-based methods, openQCM Q-1, QCM-D, Quartz Crystal Microbalance, S. aureus},
pubstate = {published},
tppubtype = {article}
}
Ensuring dairy product safety demands rapid and precise Staphylococcus aureus (S. aureus) detection. Biosensors show promise, but their performance is often demonstrated in model samples using non-native pathogens and has never been studied towards S. aureus detection in naturally contaminated samples. This study addresses the gap by directly comparing results taken with a novel piezoelectric biosensor, capable of one-step detection, with four conventional cultivation-based methods. Our findings reveal that this biosensor, based on an antifouling nanolayer-coated biochip, exhibits exceptional resistance to biofouling from unprocessed dairy products and is further capable of specific S. aureus detection. Notably, it performed comparably to Petrifilm and Baird-Parker methods but delivered results in only 30 min, bringing a substantial reduction from the 24 h required by cultivation-based techniques. Our study also highlights differences in the performance of cultivation methods when analyzing artificially spiked versus naturally contaminated foods. These findings underline the potential of antifouling biosensors as efficient reliable tools for rapid, cost-effective, point-of-care testing, enhancing fresh dairy product safety and S. aureus detection.
