openQCM – Powered by Novaetech S.r.l
Publications citing the applications of openQCM (by Novaetech S.r.l.) instruments and accessories in scientific research.
The list of scientific papers published on the most important journals showing the usage of openQCM in several scientific fields, such as thin film deposition, chemical sensors, biological research and biosensors.
Because of the large number of publications, we are reorganizing everything by subject areas. This will take some time. Thank you for your patience
Wasilewski, Tomasz; Szulczy'nski, Bartosz; Dobrzyniewski, Dominik; Jakubaszek, Weronika; Gębicki, Jacek; Kamysz, Wojciech
Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications Journal Article
In: Biosensors, vol. 12, no. 5, pp. 309, 2022.
Abstract | Links | BibTeX | Tags: biosensors, biosensors fabrication, peptides, plasma
@article{wasilewski2022development,
title = {Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications},
author = {Tomasz Wasilewski and Bartosz Szulczy'nski and Dominik Dobrzyniewski and Weronika Jakubaszek and Jacek Gębicki and Wojciech Kamysz},
url = {https://pubmed.ncbi.nlm.nih.gov/35624609/},
doi = {10.3390/bios12050309},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
journal = {Biosensors},
volume = {12},
number = {5},
pages = {309},
publisher = {MDPI},
abstract = {Cleaning a quartz crystal microbalance (QCM) plays a crucial role in the regeneration of its biosensors for reuse. Imprecise removal of a receptor layer from a transducer's surface can lead to unsteady operation during measurements. This article compares three approaches to regeneration of the piezoelectric transducers using the electrochemical, oxygen plasma and Piranha solution methods. Optimization of the cleaning method allowed for evaluation of the influence of cleaning on the surface of regenerated biosensors. The effectiveness of cleaning the QCM transducers with a receptor layer in the form of a peptide with the KLLFDSLTDLKKKMSEC-NH2 sequence was described. Preliminary cleaning was tested for new electrodes to check the potential impact of the cleaning on deposition and the transducer's operation parameters. The effectiveness of the cleaning was assessed via the measurement of a resonant frequency of the QCM transducers. Based on changes in the resonant frequency and the Sauerbrey equation, it was possible to evaluate the changes in mass adsorption on the transducer's surface. Moreover, the morphology of the QCM transducer's surface subjected to the selected cleaning techniques was presented with AFM imaging. The presented results confirm that each method is suitable for peptide-based biosensors cleaning. However, the most invasive seems to be the Piranha method, with the greatest decrease in performance after regeneration cycles (25% after three cycles). The presented techniques were evaluated for their efficiency with respect to a selected volatile compound, which in the future should allow reuse of the biosensors in particular applications, contributing to cost reduction and extension of the sensors' lifetime.},
keywords = {biosensors, biosensors fabrication, peptides, plasma},
pubstate = {published},
tppubtype = {article}
}
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