@article{millan2023degradation,

title = {Degradation kinetics of medium chain length Polyhydroxyalkanoate degrading enzyme: a quartz crystal microbalance study},

author = {Fabien Millan and Nils Hanik},

url = {https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1303267/full},

doi = {https://doi.org/10.3389/fbioe.2023.1303267},

year  = {2023},

date = {2023-12-14},

urldate = {2023-12-14},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {11},

pages = {1303267},

publisher = {Frontiers Media SA},

abstract = {This study investigates the enzymatic degradation processes of different classes of polyhydroxyalkanoates (PHAs), a group of biopolymers naturally synthesized by various microorganisms. Medium chain length PHAs (mcl-PHAs) are distinguished biopolymers due to their biodegradability and diverse material properties. Using quartz crystal microbalance measurements as a valuable tool for accurate real-time monitoring of the enzymatic degradation process, the research provides detailed kinetic data, describing the interaction between enzymes and substrates during the enzymatic degradation process. Thin films of poly-3-hydroxybutyrate (PHB) and polyhydroxyoctanoate copolymer (PHO), containing molar fractions of about 84% 3-hydroxyoctanoate and 16% 3-hydroxyhexanoate, were exposed to scl-depolymerases from Pseudomonas lemoignei LMG 2207 and recombinant mcl-depolymerase produced in Escherichia coli DH5α harboring the plasmid pMAD8, respectively. Analyses based on a heterogeneous kinetic model for the polymer degradation indicated a six-fold stronger adsorption equilibrium constant of mcl-depolymerase to PHO. Conversely, the degradation rate constant was approximately twice as high for scl-depolymerases acting on PHB. Finally, the study highlights the differences in enzyme-substrate interactions and degradation mechanisms between the investigated scl- and mcl-PHAs.},

keywords = {biodegradable polymers, degradation kinetics, depolymerase enzymes, enzymatic degradation, openQCM, openQCM Q-1, polyhydroxyalkanoates, Python, Quartz Crystal Microbalance},

pubstate = {published},

tppubtype = {article}

}