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Scientific Papers
Publications citing the applications of openQCM (by Novaetech S.r.l.) instruments and accessories in scientific research.
The list of scientific papers published on the most important journals showing the usage of openQCM in several scientific fields, such as thin film deposition, chemical sensors, biological research and biosensors.
Because of the large number of publications, we are reorganizing everything by subject areas. This will take some time. Thank you for your patience
2024
Cano-Méndez, Alan; Espinosa, Gabriel; García-Larragoiti, Nallely; Maciel-García, Pedro Antonio; Menchaca-Arredondo, Jorge Luis; Chan-Kim, Young; Reyes-Sandoval, Arturo; Viveros-Sandoval, Martha Eva
In: International Journal of Laboratory Hematology, vol. n/a, no. n/a, 2024.
Abstract | Links | BibTeX | Tags: dengue virus, non-structural 1 protein, openQCM Software, openQCM V1, platelet reactivity, platelets, zika virus
@article{https://doi.org/10.1111/ijlh.14409,
title = {Platelet Reactivity to Zika and Dengue Non-Structural Protein 1 (NS1) Assessed by Flow Cytometry, Atomic Force Microscopy, and Quartz Crystal Microbalance},
author = {Alan Cano-Méndez and Gabriel Espinosa and Nallely García-Larragoiti and Pedro Antonio Maciel-García and Jorge Luis Menchaca-Arredondo and Young Chan-Kim and Arturo Reyes-Sandoval and Martha Eva Viveros-Sandoval},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/ijlh.14409},
doi = {https://doi.org/10.1111/ijlh.14409},
year = {2024},
date = {2024-12-05},
journal = {International Journal of Laboratory Hematology},
volume = {n/a},
number = {n/a},
abstract = {ABSTRACT Background Platelets, besides being traditionally associated with hemostasis, have been recently positioned as immune cells. Alterations in platelet number and function have been reported in some viral infections. Zika virus (ZIKV) and Dengue virus (DENV) are arboviruses that encode for a non-structural protein 1 (NS1). NS1 is mainly involved in the viral replication process and can also be secreted by infected cells and has been associated with immune response evasion. The assessment of platelet reactivity against these viral agents and their proteins, through the use of different innovative technologies such as flow cytometry (FC), atomic force microscopy (AFM), and quartz crystal microbalance (QCM), will allow further study of the pathophysiology of these emerging diseases. Aim The aim of this study was to assess platelet reactivity to ZIKV and DENV NS1 protein through the use of FC, AFM, and QCM. Methods Platelet-rich plasma (PRP) was stimulated with ZIKV and DENV NS1 protein in individual assays. The expression of P-selectin and the activity of the glycoprotein IIb-IIIa, platelet activation markers, were assessed by FC, morphological changes were assessed by AFM, and interaction between NS1 protein and platelet were evaluated by QCM. Results An increased expression of P-selectin and GP IIb-IIIa activity (p < 0.001) was observed when PRP was stimulated with ZIKV and DENV NS1 proteins. AFM images showed an increase in cell size and the appearance of pseudopods upon stimulation with the viral proteins. QCM results showed a significant increase in the oscillation frequency of the quartz precoated with ZIKV or DENV NS1 when PRP was injected (p < 0.001). Conclusion FC, AFM, and QCM are techniques that can be used in the study of platelet response to viral structures such as NS1 protein, broadening the range of existing methodologies in the study of these cells. It is imperative to study platelets in arboviral infections to better understand their involvement in these diseases.},
keywords = {dengue virus, non-structural 1 protein, openQCM Software, openQCM V1, platelet reactivity, platelets, zika virus},
pubstate = {published},
tppubtype = {article}
}
ABSTRACT Background Platelets, besides being traditionally associated with hemostasis, have been recently positioned as immune cells. Alterations in platelet number and function have been reported in some viral infections. Zika virus (ZIKV) and Dengue virus (DENV) are arboviruses that encode for a non-structural protein 1 (NS1). NS1 is mainly involved in the viral replication process and can also be secreted by infected cells and has been associated with immune response evasion. The assessment of platelet reactivity against these viral agents and their proteins, through the use of different innovative technologies such as flow cytometry (FC), atomic force microscopy (AFM), and quartz crystal microbalance (QCM), will allow further study of the pathophysiology of these emerging diseases. Aim The aim of this study was to assess platelet reactivity to ZIKV and DENV NS1 protein through the use of FC, AFM, and QCM. Methods Platelet-rich plasma (PRP) was stimulated with ZIKV and DENV NS1 protein in individual assays. The expression of P-selectin and the activity of the glycoprotein IIb-IIIa, platelet activation markers, were assessed by FC, morphological changes were assessed by AFM, and interaction between NS1 protein and platelet were evaluated by QCM. Results An increased expression of P-selectin and GP IIb-IIIa activity (p < 0.001) was observed when PRP was stimulated with ZIKV and DENV NS1 proteins. AFM images showed an increase in cell size and the appearance of pseudopods upon stimulation with the viral proteins. QCM results showed a significant increase in the oscillation frequency of the quartz precoated with ZIKV or DENV NS1 when PRP was injected (p < 0.001). Conclusion FC, AFM, and QCM are techniques that can be used in the study of platelet response to viral structures such as NS1 protein, broadening the range of existing methodologies in the study of these cells. It is imperative to study platelets in arboviral infections to better understand their involvement in these diseases.
Razib, Mohd Asyraf Mohd; Mahadi, Aisyah Syafiqah; Ralib, Aliza Aini Md; Yusoff, Marmeezee Mohd; Ahmad, Farah B.
2024.
Abstract | Links | BibTeX | Tags: Biosensor, Carbon Nanotubes, openQCM Software, QCM, Quartz Crystal Microbalance, Sensing Layer
@bachelorthesis{nokey,
title = {Synthesis and Characterization of Multi-Walled Carbon Nanotube/Chitosan (Mwcnt/Cs) Composite as a Sensing Layer on Quartz Crystal Microbalance (Qcm) for Detection of Volatile Organic Compounds (Vocs)},
author = {Mohd Asyraf Mohd Razib and Aisyah Syafiqah Mahadi and Aliza Aini Md Ralib and Marmeezee Mohd Yusoff and Farah B. Ahmad},
url = {https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4727417},
year = {2024},
date = {2024-02-15},
urldate = {2024-02-15},
abstract = {Quartz crystal microbalance (QCM) is a sensor that can detect changes in nanogram mass. QCM alone is inadequate for detecting volatile organic compounds (VOCs) as it lacks sensitivity and selectivity for a gas sensor; thus, various sensing layers were suggested to be deposited. This study introduces multi-walled carbon nanotubes (MWCNT) and chitosan (CS) composite as a potential new sensing material. MWCNT has the advantage of a large surface area, improving the adsorption of gases. At the same time, CS is a natural biopolymer with a high affinity with VOCs as it is very hydrophilic. Harnessing advantages over both materials can study the response in profiling selective VOCs. Studies were conducted through the characterization of nanocomposite using Raman spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Scanning Electron Microscopy (SEM) to determine the properties of the MWCNT/CS composite on QCM toward VOCs. MWCNT/CS was prepared using glutaraldehyde as a cross-linker to form a covalent bond between MWCNT and CS via sonication. CS, MWCNT, and the composite were prepared for characterization analysis. The result of FTIR spectroscopy of MWCNT/CS showed NH2, C=O, and C-N bond at 3359.45 cm-1, 2160.51-2033.71 cm-1, and 1074.25 cm-1, respectively, showing that the functional group in CS was presented in the composite. The surface morphology of the MWCNTs/CS was detected using SEM. QCM sensor with gold electrodes was fabricated by drop-casting the composite on the working electrode of the QCM. Next, an adsorption test was conducted to study the sensitivity of the composite as the sensing layer using isopropyl alcohol (IPA, 13.1 M) as VOCs. The frequency shift of the IPA adsorption for the MWCNT/CS-based sensor was 95.2 Hz with a response time of 42s. The result shows that the MWCNT/CS can be a potential sensing layer to detect VOCs.},
keywords = {Biosensor, Carbon Nanotubes, openQCM Software, QCM, Quartz Crystal Microbalance, Sensing Layer},
pubstate = {published},
tppubtype = {bachelorthesis}
}
Quartz crystal microbalance (QCM) is a sensor that can detect changes in nanogram mass. QCM alone is inadequate for detecting volatile organic compounds (VOCs) as it lacks sensitivity and selectivity for a gas sensor; thus, various sensing layers were suggested to be deposited. This study introduces multi-walled carbon nanotubes (MWCNT) and chitosan (CS) composite as a potential new sensing material. MWCNT has the advantage of a large surface area, improving the adsorption of gases. At the same time, CS is a natural biopolymer with a high affinity with VOCs as it is very hydrophilic. Harnessing advantages over both materials can study the response in profiling selective VOCs. Studies were conducted through the characterization of nanocomposite using Raman spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, and Scanning Electron Microscopy (SEM) to determine the properties of the MWCNT/CS composite on QCM toward VOCs. MWCNT/CS was prepared using glutaraldehyde as a cross-linker to form a covalent bond between MWCNT and CS via sonication. CS, MWCNT, and the composite were prepared for characterization analysis. The result of FTIR spectroscopy of MWCNT/CS showed NH2, C=O, and C-N bond at 3359.45 cm-1, 2160.51-2033.71 cm-1, and 1074.25 cm-1, respectively, showing that the functional group in CS was presented in the composite. The surface morphology of the MWCNTs/CS was detected using SEM. QCM sensor with gold electrodes was fabricated by drop-casting the composite on the working electrode of the QCM. Next, an adsorption test was conducted to study the sensitivity of the composite as the sensing layer using isopropyl alcohol (IPA, 13.1 M) as VOCs. The frequency shift of the IPA adsorption for the MWCNT/CS-based sensor was 95.2 Hz with a response time of 42s. The result shows that the MWCNT/CS can be a potential sensing layer to detect VOCs.
