@article{TIAN2025100341,

title = {Palm-size wireless piezoelectric immune-biosensing system for rapid E. coli O157:H7 detection},

author = {Yang Tian and Lisa Kelso and Yiting Xiao and Chaitanya Pallerla and Ramesh Bist and Siavash Mahmoudi and Ziyu Liu and Haizheng Xiong and Jeyam Subbiah and Terry Howell and Dongyi Wang},

url = {https://www.sciencedirect.com/science/article/pii/S2666351125000166},

doi = {https://doi.org/10.1016/j.sintl.2025.100341},

issn = {2666-3511},

year  = {2025},

date = {2025-06-01},

urldate = {2025-01-01},

journal = {Sensors International},

volume = {6},

pages = {100341},

abstract = {Rapid and accurate detection of Escherichia coli O157:H7 is essential for ensuring public health, food safety, and environmental monitoring. Traditional detection methods are often time-consuming and require specialized laboratory infrastructure, limiting their practicality for on-site applications. In this study, we present a palm-sized, wireless piezoelectric immunosensor system designed for the swift and sensitive detection of E. coli O157:H7. The system integrates a gold-coated quartz crystal microbalance (QCM) sensor functionalized with protein A-immobilized antibodies, a polydimethylsiloxane (PDMS) microfluidic channel for efficient sample handling, and a flexible loop antenna for wireless signal transmission. Real-time data is transmitted via Bluetooth to a smartphone application, enhancing user interaction and portability. Sensitivity tests demonstrated a proportional increase in frequency shift corresponding to E. coli O157:H7 concentrations, achieving a detection limit as low as 102 CFU ml−1 in field samples. Selectivity tests confirmed minimal cross-reactivity with other bacteria, highlighting the sensor's specificity. The incorporation of immuno-nanobeads amplified the signal, enhancing the system's ability to detect low bacterial concentrations. The portable, self-powered system offers a practical solution for rapid, on-site pathogen detection, addressing critical needs in food safety and environmental monitoring. By enabling timely and accurate detection of contaminants, this technology has the potential to significantly impact public health by reducing the incidence of foodborne illnesses, safeguarding environmental resources, and enhancing overall safety and health outcomes.},

keywords = {Food safety, Immuno-nanobeads, immunosensor, Microfluidic, openQCM sensors, Pathogen detection, Piezoelectric sensor, Wireless biosensor},

pubstate = {published},

tppubtype = {article}

}

@article{saffari2023signal,

title = {Signal amplification of a quartz crystal microbalance immunosensor by gold nanoparticles-polyethyleneimine for hepatitis B biomarker detection},

author = {Zahra Saffari and Reza Ahangari Cohan and Mina Sepahi and Mahdi Sadeqi and Mehdi Khoobi and Mojtaba Hamidi Fard and Amir Ghavidel and Fahimeh Bagheri Amiri and Mohammad Reza Aghasadeghi and Dariush Norouzian},

url = {https://www.nature.com/articles/s41598-023-48766-2},

doi = {https://doi.org/10.1038/s41598-023-48766-2},

year  = {2023},

date = {2023-12-09},

urldate = {2023-12-09},

journal = {Scientific Reports},

volume = {13},

number = {1},

pages = {21851},

publisher = {Nature Publishing Group UK London},

abstract = {The procedures currently used for hepatitis B (HB) detection are not suitable for screening, clinical diagnosis, and point-of-care testing (POCT). Therefore, we developed and tested a QCM-based immunosensor by surface modification with AuNP-PEIs to amplify the signal and provide an oriented-immobilization surface. The AuNP-PEIs were characterized by ICP-Mass, UV/Vis, DLS, FE-SEM, and ATR-FTIR. After coating AuNP-PEIs on the gold electrode surface, anti-HBsAg antibodies were immobilized using NHS/EDC chemistry based on response surface methodology (RSM) optimization. The efficiency of the immunosensor was assessed by human sera and data were compared to gold-standard ELISA using receiver-operating-characteristic (ROC) analysis. FE-SEM, AFM, EDS, and EDS mapping confirmed AuNP-PEIs are homogeneously distributed on the surface with a high density and purity. After antibody immobilization, the immunosensor exhibited good recognition of HBsAg with a calibration curve of ∆F =  − 6.910e-7x + 10(R2 = 0.9905), a LOD of 1.49 ng/mL, and a LOQ of 4.52 ng/mL. The immunosensor yielded reliable and accurate results with a specificity of 100% (95% CI 47.8–100.0) and sensitivity of 100% (95% CI 96.2–100.0). In conclusion, the fabricated immunosensor has the potential as an analytic tool with high sensitivity and specificity. However, further investigations are needed to convert it to a tiny lab-on-chip for HB diagnosis in clinical samples.},

keywords = {biomarker, hepatitis B, immunosensor, nanoparticles-polyethyleneimine, openQCM, openQCM sensors, QCM},

pubstate = {published},

tppubtype = {article}

}