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Publications citing the applications of openQCM (by Novaetech S.r.l.) instruments and accessories in scientific research.
The list of scientific papers published on the most important journals showing the usage of openQCM in several scientific fields, such as thin film deposition, chemical sensors, biological research and biosensors.
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Saffari, Zahra; Sepahi, Mina; Ahangari-Cohan, Reza; Khoobi, Mehdi; Hamidi-Fard, Mojtaba; Ghavidel, Amir; Aghasadeghi, Mohammad Reza; Norouzian, Dariush
A quartz crystal microbalance biosensor based on polyethylenimine-modified gold electrode to detect hepatitis B biomarker Journal Article
In: Analytical Biochemistry, pp. 114981, 2022.
Abstract | Links | BibTeX | Tags: biosensors, Hepatitis B Biomarker, Polyethylenimine, Quartz Crystal Microbalance
@article{saffari2022quartz,
title = {A quartz crystal microbalance biosensor based on polyethylenimine-modified gold electrode to detect hepatitis B biomarker},
author = {Zahra Saffari and Mina Sepahi and Reza Ahangari-Cohan and Mehdi Khoobi and Mojtaba Hamidi-Fard and Amir Ghavidel and Mohammad Reza Aghasadeghi and Dariush Norouzian},
url = {https://www.sciencedirect.com/science/article/abs/pii/S0003269722004419},
doi = {https://doi.org/10.1016/j.ab.2022.114981},
year = {2022},
date = {2022-01-01},
urldate = {2022-01-01},
journal = {Analytical Biochemistry},
pages = {114981},
publisher = {Elsevier},
abstract = {Biomarkers-based QCM-biosensors are suitable tools for the label-free detection of infectious diseases. In the current study, a QCM-biosensor was developed for the detection of HBsAg. Briefly, anti-HBsAg antibodies were covalently bound to the primary amines after PEI and thiolated-PEI surface modifications of gold-electrode. After RSM optimization, the statistical analysis revealed no significant difference between the immobilization yields of modified layers. Therefore, the PEI-modified QCM-biosensor was selected for further analysis. The PEI-surface was evaluated by FESEM, AFM, ATR-FTIR, and CA measurement. The surface hydrophilicity and its roughness were increased after PEI-coating. Also, FTIR confirmed the PEI-layering on the gold-surface. RSM optimization increased the antibody immobilization yield up to 80%. The QCM-biosensor showed noteworthy results with a wide dynamic range of 1–1 × 103 ng/mL, LOD of 3.14 ng/mL, LOQ of 9.52 ng/mL, and detection capability in human-sera, which were comparable with the ELISA. The mean accuracy of the QCM-biosensor was obtained at 91% when measured by the spike recovery test using human-sera. The biosensor was completely regenerated using 50 mM NaOH and 1% SDS. The benefits provided by the developed biosensor such as broad dynamic range, sensitivity, selectivity, stability, regenerate ability, and low cost suggest its potential application for the non-invasive and timely monitoring of HBV-biomarker.},
key = {Hepatitis B Biomarker, Biosensor, Quartz crystal microbalance, Polyethylenimine, Gold electrode},
keywords = {biosensors, Hepatitis B Biomarker, Polyethylenimine, Quartz Crystal Microbalance},
pubstate = {published},
tppubtype = {article}
}
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