# Site-specific imprinting of dengue virus non-structural 1 antigen on a polydopamine-based sensing film for early detection and prognosis of dengue

> Source page: https://openqcm.com/scientific-papers/site-specific-imprinting-of-dengue-virus-non-structural-1-antigen-on-a-polydopamine-based-sensing-film-for-early-detection-and-prognosis-of-dengue
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- Authors: Hui Jean Lim, Tridib Saha, Chien Wei Ooi
- Journal: Talanta
- Year: 2024
- Volume: 268
- Pages: 125376
- DOI: 10.1016/j.talanta.2023.125376
- Instrument used: openQCM Q-1

## Abstract

Serum levels of dengue virus (DENV) non-structural 1 (NS1) antigen can serve as a valuable prognostic indicator of severe dengue infections. A quartz crystal microbalance (QCM)-based biosensor with a biomimetic recognition element was designed to quantitatively detect DENV NS1 as an early disease biomarker. To mitigate the reliance on costly viral antigens during the molecular imprinting process, a synthetic peptide mimicking a DENV NS1 epitope was used as a surrogate template for the synthesis of an epitope-imprinted polydopamine (EMIPDA) sensing film on the biosensor surface. The maximal frequency shift for DENV NS1 was obtained with an EMIPDA film synthesised using 5 mg mL⁻¹ of dopamine monomer and 0.5 mg mL⁻¹ of peptide template. The EMIPDA-QCM biosensor achieved low detection and quantitation limits of 0.091 μg mL⁻¹ and 0.436 μg mL⁻¹, respectively, allowing acute-phase detection of dengue and prognosis of the disease progression. The EMIPDA-QCM biosensor exhibited remarkable selectivity with up to 68-fold larger frequency responses towards DENV NS1 compared to a major serum protein. The site-specific imprinting approach not only enhanced the biosensing performance but also enabled a 26-fold cost reduction for biosensor functionalisation, providing a cost-effective strategy for label-free biosensing of the dengue biomarker via the biopolymer film.

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